Afinitas Lutein dari Ekstrak Bunga Marigold sebagai Potensi Terapi Kanker Tiroid pada Protein PPARG, RXRS, DAN MAP2K2 melalui Docking Senyawa
DOI:
https://doi.org/10.33830/saintek.v1i1.10034.2024Kata Kunci:
Tagetes erecta L., dekok, docking senyawa, Autodock vina, Spectrophotometer uv-visAbstrak
Penelitian ini bertujuan untuk mengoptimasi ekstraksi Lutein dari bunga marigold (Tagetes erecta L.) menggunakan metode dekokta dan mengevaluasi aktivitas antikankernya melalui studi docking senyawa. Bunga marigold diekstrak menggunakan metode dekokta sebanyak tujuh kali pada suhu 90°C, menghasilkan ekstrak serbuk dengan kadar Lutein rata-rata 2,42%. Kandungan Lutein dianalisis menggunakan spektrofotometer UV-Vis pada panjang gelombang 446 nm. Aktivitas antikanker Lutein dievaluasi melalui docking senyawa menggunakan perangkat lunak AutoDock Vina. Hasil docking menunjukkan bahwa Lutein memiliki afinitas tinggi terhadap protein-protein yang terkait dengan kanker tiroid, seperti MAP2K2, RXRA, RXRB, RXRG, dan PPARG, dengan nilai binding affinity yang rendah, menandakan ikatan yang kuat dan akurat. Studi ini menunjukkan potensi Lutein sebagai agen antikanker tiroid, namun penelitian lebih lanjut diperlukan untuk memvalidasi mekanisme Lutein melalui studi in silico, in vitro dan in vivo.
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