PENAPISAN AKTINOMISETES YANG BERSIFAT ANTAGONIS TERHADAP PENYAKIT LAYU BAKTERI TANAMAN CABE
Keywords: actinomisetes, antagonist, Ralstonia solanacearum
Abstract
Ralstonia solanacearum can cause droop bacteria desease in chilli plants which resulted in productivity’s decreasing. Chemical subtances commonly used to eliminate the bacteria is costly and have negative effect on environment. This research aims to eliminate the varied actinomisetes and to get antagonistics actinomisetes towards the droop bacteria desease of chilli plants. The Botanical forest in Baturaden was chosen because has unique and high diversity microorganism that has characteristic antagonism that pressure growth of other antagonistic’s because of antimicrobe. A number of 18 actinomisetes isolat were successfully isolated and then rejuvenated based on morphology, colours, and shape of the colonies. A characterization of morphology trait towards target bacteria had also been conducted. Six of the 18 actinomisetes isolat have antagonist characteristics towards target bacteria’s growth. Isolat Btrd II.g and Btrd IV.l have the highest resistor activity as indicated by a 6 and 5 mm crystal clear zone. It indicated that actinomisetes isolat Btrd II.g and Btrd IV.l have relatively high antagonist characteristics. Btrd II.g and Btrd IV.l are potential to be used as an environmental friendly way to eliminate droop bacteria desease in chilli plants.
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References
Chamberlain, K. & Crawford DL. (2000). Thatch biodegradation and antifungal activities of two lignocellulolytic Streptomyces strain in laboratory cultures an in golf green turf grass. Can J. Microbiolol 46: 550-558.
Cook, R.J. & Baker, K.F. (1996). The nature and practice of biological control of plant pathogens. Ed. ke-3. St. Paul Minnesota. APS.
Dirjen Bina Produksi Hortikultura. (2002). Informasi hortikultura dan aneka tanaman hortikultura. Jakarta.
Djafaruddin. (2000). Dasar-dasar pengendalian penyakit tanaman. Jakarta: Bumi Aksara.
Holt, J.G., Krieg, N.R., Sneath, P.H.A.,Staley, J.T. & Williams, S.T. (1994). Bergey’s manual of determinative bacteriology. Ed. ke-9. USA: Williams & Wilkins.
Hwang, B.K., Lee, J.Y., Kim, B.S., & Moon, S.S. (1996). Isolation, structure elucidation, and antifungal activity of manumycin-type antibiotic from actinomycetes. Agric Food Chem 44: 3653-3657.
Hwang, B.K., Lim, S.W., Kim, B.S., Lee, J.Y. & Moon, S.S. (2001). Isolation and in vivo and in vitro antifungal activity of phenylacetic acid and sodium phenylacetate from Streptomyces humidus. Appl Environ Microbiol 67:3739-3745.
Madigan, M.T., Martinko, J.M., & Parker, J. (2000). Brock: Biology of microorganisms. New Jersey American: Prentice Hall.
Mc-Manus P.S., Stockwell V.O. (2001). Antibiotic use for plant disease management in the United States. Plant Health Progress.
Miyadoh, S. & Otoguro, M. (2004). Introduction of actinomycetes. Workshop on isolation methods and classification of Actinomycetes. Bogor: Biotechnology Center LIPI.
Neeno-Eckwall E.C., Kinkel L.L., Schottel J.L. (2001). Competition and antibiosis in the biological control of Potato scab. Can J Microbiol 47:332-340.
Semangoen, H. (1994). Penyakit-penyakit tanaman hortikultura. Yogyakarta: Gadjah Mada Univ Pr.
Suriawiria, U. (1973). Mikroflora penghasil aktivitas anti bakteri di dalam sampel tanah dari beberapa tempat di Jawa Barat. Acta Paramacautica 4 (1):10-17.
Syefiyanah. (2000). Antibiosis Isolat Streptomyces sp. terhadap patogen kedelai Bacillus subtilis dan Xanthomonas campestris pv. glycine. Skripsi. Bogor: Fakultas Matematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor.
Yuan, W.M. & Crawford, D.L. (1995). Characterization of Streptomyces lydicus WYEC108 as a potential biocontrol agent against fungal root and seed rots. Appl Environ Microbiol 61:3119-3128.