Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia)

Penulis

  • Susila Susila Badan Riset dan Inovasi Nasional
  • I Putu Gede P. Damayanto Badan Riset dan Inovasi Nasional (BRIN)
  • Kusumadewi Sri Yulita Badan Riset dan Inovasi Nasional (BRIN)
  • Nawwall Arrofaha Badan Riset dan Inovasi Nasional (BRIN)

DOI:

https://doi.org/10.33830/manilkara.v2i2.7517.2024

Kata Kunci:

CTAB, CITES, ektraksi DNA, kit, PCR

Abstrak

Sonokeling (Dalbergia latifolia) merupakan tumbuhan berkayu keras, tahan terhadap serangan rayap, kayunya memiliki serat dan tekstur bermotif indah, sehingga banyak dimanfaatkan untuk furniture pada katagori mewah yang bernilai ekonomi tinggi. Menurut CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora), status perdagangan sonokeling termasuk dalam appendix II. Dalam perdagangan kayu sonokeling, baik pada tingkat pasar domestik maupun internasional, untuk mengidentifikasi asal-usul kayu sonokeling tersebut relatif banyak ditemukan kendala dan kesulitan. Referensi tentang identitas kayu berdasarkan lokasi asal sangat penting dibangun sebagai salah satu upaya untuk mencegah perdagangan liar, yaitu dengan cara menggunakan pendekatan identifikasi berbasis molekuler (sidik DNA). Pada isolasi DNA kayu sonokeling diperlukan teknis khusus karena karakteristik kayunya keras. Penelitian ini bertujuan untuk membandingkan teknik isolasi DNA dari kayu dan daun sonokeling menggunakan protokol CTAB (Cetyl Trimethyl Ammonium Bromide) dan protokol kit komersial. Penelitian menggunakan metode identifikasi berbasis biologi molekuler. Hasil PCR menunjukkan bahwa protokol isolasi DNA menggunakan kit komersial mengungguli protokol CTAB untuk sampel daun dan kayu. Isolasi DNA dari sampel kayu lebih sukar dilakukan daripada sampel daun. Secara keseluruhan, protokol terbaik untuk isolasi DNA dari daun dan kayu sonokeling adalah dengan menggunakan kit komersial.

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Diterbitkan

2024-02-29

Cara Mengutip

Susila, S., I Putu Gede P. Damayanto, Kusumadewi Sri Yulita, & Nawwall Arrofaha. (2024). Perbandingan Teknik Isolasi DNA pada Daun dan Kayu Sonokeling (Dalbergia latifolia). MANILKARA: Journal of Bioscience, 2(2), 69–81. https://doi.org/10.33830/manilkara.v2i2.7517.2024

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