Antibacterial Activity of Ethanolic Extract Siwak Stem (Salvadora persica) Against Staphylococcus aureus ATCC 25923

Penulis

  • Nisa’ Nur Sholikhah Department of Medical Laboratory Technology, Faculty of Health Science, Setia Budi University
  • Andang Arif Wibawa Department of Medical Laboratory Technology, Faculty of Health Science, Setia Budi University
  • Rizal Maarif Rukmana National Research and Innovation Agency (BRIN) of Indonesia

DOI:

https://doi.org/10.33830/manilkara.v1i2.4095.2023

Kata Kunci:

Antibacterial, Salvadora persica stem, Etanol, Staphylococus aureus

Abstrak

Infeksi dapat disebabkan oleh bakteri dan salah satunya adalah Staphylococcus aureus. Pengobatan infeksi dapat menggunakan tumbuhan alami seperti tanaman Siwak (Salvadora persica). Batang tanaman Siwak (Salvadora persica) mengandung Saponin, Alkaloid, Tanin, dan Fenol. Penelitian ini untuk mengetahui aktivitas antibakteri batang Salvadora persica terhadap pertumbuhan Staphylococcus aureus ATCC 25923. Metode ekstraksi menggunakan maserasi dengan pelarut adalah etanol. Golongan senyawa ekstrak diidentifikasi menggunakan berbagai pereaksi kimia. Ekstrak batang Salvadora persica dibuat dengan variasi konsentrasi 6,25%, 12,5%, 25%, dan 50% dengan menggunakan pengencer dimetil sulfoksida 2%  (DMSO). Klorheksidin 0,2% sebagai kontrol positif, dan DMSO 2% sebagai kontrol negatif. Uji aktivitas antibakteri dilakukan  dengan metode difusi. Analisis data menggunakan statistika yaitu dengan uji ANOVA. Hasil ekstrak etanol batang siwak mengandung golongan senyawa: saponin, alkaloid, tanin, dan fenol. Hasil penelitian menunjukkan bahwa ekstrak batang Salvadora persica memiliki aktivitas antibakteri terhadap Staphylococcus aureus ATCC 25923. Uji aktivitas antibakteri terhadap Staphylococcus aureus dari kultur laboratorium menunjukkan rata-rata diameter zona hambat terbesar pada konsentrasi 50% dengan diameter 20,5 mm dan zona hambat terkecil pada konsentrasi 6,5 mm. Konsentrasi 25% dengan diameter 14 mm.

 

Kata Kunci : Antibakteri, Batang Salvadora persica, Etanol, Staphylococcus aureus

Referensi

Abdallah, E. M., & Al-Harbi, K. A. (2015). Phytochemical screening and antibacterial activity of crude aqueous and ethanol extracts of Salvadora persica L. stem (Miswak) from Saudi Arabia. The Journal of Phytopharmacology, 4(5), 243–247. https://doi.org/10.31254/phyto.2015.4501

Abhary, M., & Al-Hazmi, A.-A. (2016). Antibacterial activity of Miswak ( Salvadora persica L.) extracts on oral hygiene . Journal of Taibah University for Science, 10(4), 513–520. https://doi.org/10.1016/j.jtusci.2015.09.007

Agustin, B. A., Puspawaty, N., & Rukmana, R. M. (2018). Aktivitas Antibakteri Kombinasi Ekstrak Etanolik Daun Beluntas (Pluchaea indica Less.) dan Meniran (Phyllanthus niruri L.) terhadap Bakteri Staphylococcus aureus. Biomedika, 11(2), 79–87. https://doi.org/10.31001/biomedika.v11i2.425

Al-Ayed, M. S. Z., Asaad, A. M., Qureshi, M. A., Attia, H. G., & Almarrani, A. H. (2016). Antibacterial activity of Salvadora persica L. (Miswak) extracts against multidrug resistant bacterial clinical isolates. Evidence-Based Complementary and Alternative Medicine, 2016. https://doi.org/10.1155/2016/7083964

Amal, R. A. N. M. S. (2018). AKTIVITAS ANTIBAKTERI KAYU SIWAK (Salvadora persica) FRAKSI ETER TERHADAP BAKTERI Staphylococcus aureus SECARA IN VITRO. Pharmaceutical Journal of Islamic Pharmacy, 2(1), 16. https://doi.org/10.21111/pharmasipha.v2i1.2132

Djamal, J. M., P, J. M., & Rukmana, R. M. (2020). Aktivitas sitotoksik dan antiangiogenesis umbi mentimun papasan (Coccinia grandis L.Voight) terhadap sel kanker hela yang diinduksi protein bFGF. Riset Informasi Kesehatan, 9(1), 51. https://doi.org/10.30644/rik.v9i1.309

Hidanah, S., Sabdoningrum, E. K., Wahjuni, R. S., & Arimbi, A. (2017). Implementation of Meniran Extract (Phyllanthus Niruri Linn) on the Performance of Broiler Chickens Infected by Mycoplasma gallisepticum Caused Chronic Respiratory Disease. KnE Life Sciences, 3(6), 296. https://doi.org/10.18502/kls.v3i6.1138

Hussen, S., Mulatu, G., & Yohannes Kassa, Z. (2019). Prevalence of Shigella species and its drug resistance pattern in Ethiopia: A systematic review and meta-analysis. Annals of Clinical Microbiology and Antimicrobials, 18(1), 1–11. https://doi.org/10.1186/s12941-019-0321-1

Kamil, V. S., Munawir, A., & Dewi, R. (2013). Efek Antibakterial Ekstrak Etanol Siwak ( Salvadora persica ) terhadap Pertumbuhan Bakteri Porphyromonas gingivalis ( The Antiabacterial Effect of Ethanolic Extract of Salvadora persica on Growth of Porphyromonas gingivalis ). Skrips, 1–4. https://repository.unej.ac.id/

McCormack, M. G., Smith, A. J., Akram, A. N., Jackson, M., Robertson, D., & Edwards, G. (2015). Staphylococcus aureus and the oral cavity: An overlooked source of carriage and infection? American Journal of Infection Control, 43(1), 35–37. https://doi.org/10.1016/j.ajic.2014.09.015

Mohammed, N. A. (2012). Effect of Nigella Sativa L. extracts against Streptococcus mutans and Streptococcus mitis in Vitro. J Bagh College Dentistry, 24(3), 154–157.

Noor, A. S., Triatmoko, B., & Nuri, N. (2020). Uji Aktivitas Antibakteri Ekstrak Metanol dan Fraksi Daun Kenikir (Cosmos caudatus Kunth) terhadap Salmonella typhi. Pustaka Kesehatan, 8(3), 177. https://doi.org/10.19184/pk.v8i3.13008

Pantosti, A. (2012). Methicillin-resistant Staphylococcus aureus associated with animals and its relevance to human health. Frontiers in Microbiology, 3(APR), 1–12. https://doi.org/10.3389/fmicb.2012.00127

Prastiyanto, M. E., Rukmana, R. M., Saraswati, D. K., Darmawati, S., Maharani, E. T. W., & Tursinawati, Y. (2020). Anticancer potential of methanolic extracts from pleurotus species on raji cells and antibacterial activity against methicillin-resistant staphylococcus aureus. Biodiversitas, 21(12), 5644–5649. https://doi.org/10.13057/biodiv/d211221

Robertson, D., & Smith, A. J. (2009). The microbiology of the acute dental abscess. Journal of Medical Microbiology, 58(2), 155–162. https://doi.org/10.1099/jmm.0.003517-0

Rukmana, R. M. (2015). Aktivitas Antibakteri dari Ekstrak Etanolik Daun Kumis Kucing. Biomedika, 8(2).

Rukmana, R. M., Nugroho, R. B., & Wisnumurti, D. A. (2020). UJI AKTIVITAS ANTIBAKTERI EKSTRAK ETANOLIK UMBI AKAR BATU ( Coccinia grandis L . Voight ) TERHADAP BAKTERI SALMONELLA INDUSTRI Budi Nugroho SURABAYA ,. Bioeksperimen, 5(1), 133–140. https://doi.org/10.23917/bioeksperimen.v5i1.2795

Rukmana, R. M., Nugroho, R. B., Wisnumurti, D. A., & Wibawa, A. A. (2019). Uji aktivitas antibakteri ekstrak etanolik umbi mentimun papasan (Coccinia grandis L.Voigt) terhadap Shigella dysenteriae dan Staphylococcus aureus. Riset Informasi Kesehatan, 8(2), 91. https://doi.org/10.30644/rik.v8i2.233

Rukmana, R. M., Soesilo, N. P., & Pratiwi, R. (2016). The Effect of Ethanolic Extract of Black and White Rice Bran ( Oryza sativa L .) on Cancer Cells. 21(1), 63–69. https://doi.org/10.22146/ijbiotech.26814

Seran, A. A., Peranginangin, J. M., & Rukmana, R. M. (2020). Cytotoxic Activity and Antiangiogenesis of Extract Fraction of Coccinia grandis (L.) Voigt) Base Towards T47D Breast Cancer Cells and Chorio Allantoic Membrane (CAM) of Chicken Embryo Induced by bFGF. Jurnal Ilmiah Sains, 20(1), 37. https://doi.org/10.35799/jis.20.1.2020.27032

Shilpa, V., Muddukrishnaiah, K., Thavamani, Bs., Dhanapal, V., Arathi, K., Vinod, K., & Sreeranjini, S. (2018). In vitro immunomodulatory, antifungal, and antibacterial screening of Phyllanthus niruri against to human pathogenic microorganisms. Environmental Disease, 3(3), 63. https://doi.org/10.4103/ed.ed_9_18

Suryani, L., & Astuti, Y. (2016). Uji Kadar Hambatan Minimal Ekstrak Batang Siwak (Salvadora persica) terhadap Staphylococcus aureus secara In Vitro. Mutiara Medika: Jurnal Kedokteran Dan Kesehatan, 7(1), 7–12. http://journal.umy.ac.id/index.php/mm/article/view/1690

Turahman, T. (2019). Aktivitas Antibakteri Ekstrak Dan Fraksi Herba Kemangi (Ocimum Sanctum L) Terhadap Staphylococcus Aureus Dan Pseudomonas Aeruginosa. Jurnal Farmasi Indonesia, 16(2), 90–97. https://doi.org/10.31001/jfi.v16i2.596

Unduhan

Diterbitkan

2023-02-01

Cara Mengutip

Nisa’ Nur Sholikhah, Andang Arif Wibawa, & Rukmana, R. M. (2023). Antibacterial Activity of Ethanolic Extract Siwak Stem (Salvadora persica) Against Staphylococcus aureus ATCC 25923. MANILKARA: Journal of Bioscience, 1(2), 55–61. https://doi.org/10.33830/manilkara.v1i2.4095.2023

Terbitan

Vol 1 No 2 (2023): Februari

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