Dampak Lama Penyimpanan Arsip Sampel Formalin-Fixed Paraffin-Embedded (FFPE) dengan Konsentrasi Ribonucleid acid (RNA) yang Diekstraksi Menggunakan Kit Komersial

Penulis

  • Nur Eka Wiraditya Patologi Anatomi FKKMK Universitas Gadjah Mada

DOI:

https://doi.org/10.33830/manilkara.v1i2.4442.2023

Kata Kunci:

Formalin-Fixed Paraffin-Embedded (FFPE), RNA, Konsentrasi RNA

Abstrak

Formalin fixation and paraffin embedding (FFPE) merupakan salah satu metode penyimpananrsip sampel dalam jangka waktu bertahun-tahun. Penyimpanan sampel dalam jangka waktu yang lama memungkinkan terjadinya penurunan kualitas RNA pada sampel. Penelitian iniengetahui perbedaan konsentrasi RNA pada arsip sampel 2019 dan 2020 dengan satuan ng/L. Penelitian menggunakan sampel yang diambil dari arsip FFPE Rumah Sakit (RS) X sebanyak 20 sampel. Rincian sampel adalah 10 (sepuluh) sampel pada tahun 2019 dan 10 (sepuluh) sampel pada tahun 2020. Penelitian ini dilakukan pada tahun 2021 di laboratorium Patologi Anatomik FK-KMK UGM. Penelitian ini menunjukkan dampak signifikan terhadap lama penyimpanan arsip sampel FFPE ditunjukkan dengan penurunan konsentrasi RNA antara tahun 2019 dan 2020 dengan perbedaan konsentrasi rata-rata 12,3 ng/L.

Referensi

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Unduhan

Diterbitkan

2023-04-04

Cara Mengutip

Wiraditya, N. E. (2023). Dampak Lama Penyimpanan Arsip Sampel Formalin-Fixed Paraffin-Embedded (FFPE) dengan Konsentrasi Ribonucleid acid (RNA) yang Diekstraksi Menggunakan Kit Komersial. MANILKARA: Journal of Bioscience, 1(2), 80–85. https://doi.org/10.33830/manilkara.v1i2.4442.2023

Terbitan

Vol 1 No 2 (2023): Februari

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