Effective technology integration in biology teaching requires competent educators, yet many face challenges, highlighting the need for relevant professional development. This paper details the foundational phase of a larger Research and Development (R&D) project aimed at developing and evaluating an e-training program for secondary biology teachers in Samarinda, Indonesia, via the Universitas Terbuka platform. Specifically, this work focuses on the initial design of instruments intended to understand teacher needs and context, aligning with the analysis stage of the Dick & Carey model. We describe the design process for three preliminary instruments: a survey questionnaire for mapping general needs, a semi-structured interview guide for in-depth qualitative insights, and an observation protocol for contextual analysis. Informed by literature on digital pedagogy and needs assessment principles, these instruments are crucial for ensuring the subsequent e-training program is contextually relevant and effectively targeted. Importantly, the instruments presented herein are initial designs and await validation. This paper contributes by outlining the systematic methodological process for developing these essential analysis tools, a critical first step before subsequent validation, data collection, and program development phases.