Added Value Improvement by Cassava Dregs and Rice Bran as Substrates for Citric Acid Production

  • Kirana Sanggrami Sasmitaloka Balai Besar Pengujian Standar Instrumen Pascapanen Pertanian
  • Maritsya Dita Kurnia Putri Balai Besar Pengujian Standar Instrumen Pascapanen Pertanian

DOI:

https://doi.org/10.33830/fsj.v3i2.5106.2023

Keywords:

Aspergillus niger, cassava dregs, citric acid, rice bran, solid state fermentation

Abstract

Worldwide demand for citric acid is increasing faster than production, so a more economical process is needed. Aspergillus niger is capable of producing citric acid from agroindustrial by-products. Utilization of cassava dregs and rice bran as substrates in citric acid fermentation can increase the added value of these agroindustrial by-products. This research aims to study the utilization of cassava dregs and rice bran as citric acid production substrates by Aspergillus niger on solid state fermentation and the characteristics of the citric acid they produce. The study was designed using a completely randomized design with treatment combinations between the percentage of inoculum volume w/v (x1: 2.5%; x2: 5%; and x3: 7.5%) and incubation time (y1: 1 day; y2: 2 days; y3: 3 days; y4: 4 days; y5: 5 days; y6: 6 days; and y7: 7 days), repeated four times. The addition of Aspergillus niger inoculum volume as much as 5% w/v with an incubation time of five days can produce optimal citric acid. In this combination of treatments, a pH value of 2.65, a total acid of 13.91 g/L, and a citric acid content of 9.74 g/L were produced.

References

Abbas, N., Safdar, W., Ali, S., Choudhry, S., & Ilahi, S. (2016). Citric Acid Production from Aspergillus niger using Banana Peel. International Journal of Scientific & Engineering Research, 7(1), 1580–1583.

Adeoye, A. ., Lateef, A., & Gueguim-Kana, E. . (2015). Optimization of Citric Acid Production Using A Mutant Strain of Aspergillus niger On Cassava Peel Substrate. Biocatalysis and Agricultural Biotechnology, 4(4), 568–574. https://doi.org/10.1097/JU.0000000000002945

Apriyani, M., & Sedyadi, E. (2015). Sintesis dan Karakterisasi Plastik Biodegradable dari Pati Onggok Singkong dan Ekstrak Lidah Buaya (Aloe vera) dengan Plasticizer Gliserol. Jurnal Sains Dasar, 4(2), 145–152.

Ariyani, S.B., Asmawit, Utomo, P.P. Optimasi Waktu Inkubasi Produksi Enzim Selulase oleh Aspergillus niger Menggunakan Fermentasi

Substrat Padat. Biopropal Industri, 5(2) 61-67. http://202.47.80.55/biopropal/article/view/825/736

Auta, H. S., Abidoye, K. T., Tahir, H., Ibrahim, A. D., & Aransiola, S. A. (2014). Citric Acid Production by Aspergillus niger Cultivated on Parkia biglobosa Fruit Pulp. International Scholarly Research Notices, 2014, 1–8. https://doi.org/10.1155/2014/762021

Behera, B. C. (2020). Citric acid from Aspergillus niger: a comprehensive overview. Critical Reviews in Microbiology, 46(6), 727–749. https://doi.org/10.1080/1040841X.2020.1828815

Carolina, A., Sidik, A., Maksum, I. P., Rachman, S. D., Safari, A., & Ishmayana, S. (2015). Fermentasi Biak Rendam Molases dengan Aspergillus niger untuk Produksi Asam Sitrat. In Chimica et Natura Acta (Vol. 3, Issue 1, pp. 25–29). https://doi.org/10.24198/cna.v3.n1.9171

Chergui, D., Akretche-kelfat, S., Lamoudi, L., Al-Rshaidat, M., Boudjelal, F., & Ait-amar, H. (2021). Optimization of Citric Acid Production by Aspergillus niger Using Two Downgraded Algerian Date Varieties. Saudi Journal of Biological Sciences, 28(12), 7134–7141. https://doi.org/https://doi.org/10.1016/j.sjbs.2021.08.013

Fibriyani, D., Arinta, F., & Kusumaningtyas, R. D. (2017). Pengolahan Onggok Singkong Sebagai Plastik Biodegradable Menggunakan Plasticizer Gliserin Dari Minyak Jelantah. Jurnal Aplikasi Teknologi Pangan, 6(2), 74–77. https://doi.org/10.17728/jatp.195

Kareem, S. ., & Rahman, R. . (2011). Utilization of Banana Peels for Citric Acid Production by Aspergillus niger. Agriculture and Biology Journal of North America, 4(4), 384–387. https://doi.org/10.5251/abjna.2013.4.4.384.387

Kiramang, K. (2011). Berat Badan Akhir, Konversi Ransum dan Income Over Feed and Chick Cost Ayam Broiler dengan Pemberian Ransum Komersil. Jurnal Teknosains. 5 (1), 15-25. http;//journal.uin-alaudin.ac.id/index.php/teknosains/article/view/163

Komari, Rozzana, R., Mahmud, M.K., 1996. Akumulasi Lemak pada Rhizopus SP melalui Proses Fermentasi Padat. Center for Research and Development of Nutrition and Food, NIHRD.

Kusmarwati, A., Heruwati, E. S., Utami, T., & Rahayu, E. S. (2011). Pengaruh Penambahan Pediococcus Acidilactici F-11 sebagai Kultur Starter terhadap Kualitas Rusip Teri (Stolephorus Sp.). Jurnal Pascapanen Dan Bioteknologi Kelautan Dan Perikanan, 6(1), 13–26. https://doi.org/10.15578/jpbkp.v6i1.84

Marbun, F. G. I., Wiradimadja, R., & Hernaman, I. (2018). Pengaruh Lama Penyimpanan Terhadap Sifat Fisik Dedak padi. Jurnal Ilmiah Peternakan Terpadu, 6(November), 163–166.

Musita, N. (2018). Kajian Sifat Fisikokimia Tepung Onggok Industri Besar Dan Industri Kecil. Majalah Teknologi Agro Industri, 10(1), 19–24. https://doi.org/10.46559/tegi.v10i1.3990

Naim, Nurlia. Pemanfaatan Bekatul Sebagai Media Alternatif untuk Pertumbuhan Aspergillus Sp. Media Analisis Kesehatan, 7(2), 1-6. https://anakes.poltekkes-mks.ac.id/wp-content/uploads/2018/09/1-Hj-Nurlia-Naim-Jurnal-Nov-2016.pdf

Prihastuti, P., Aji, B. P., Fitriani, D. S., Maharani, F., & Hartati, I. (2021). Optimasi Asam Sitrat Limbah Batang Pisang (Musa Paradisiaca L.) dengan Metode Kultivasi Cair. Prosiding Seminar Nasional Sains Dan Teknologi Ke-11, 1(1), 67–71.

Roukas, T., & Kotzekidou, P. (2020). Pomegranate Peel Waste: A New Substrate for Citric Acid Production by Aspergillus niger in Solid-State Fermentation Under Non-Aseptic Conditions. Environmental Science and Pollution Research, 27(12), 13105–13113. https://doi.org/10.1007/s11356-020-07928-9

Sari, M., Warji, Novita, D. D., & Tamrin. (2013). Mempelajari Karakteristik Tepung Onggok Pada Tiga Metode Pengeringan Yang Berbeda. Jurnal Teknik Pertanian Lampung, 2(1), 43–48.

Sasmitaloka, K. S. (2017). Produksi Asam Sitrat oleh Aspergillus niger pada Kultivasi Media Cair. Jurnal Integrasi Proses, 6(3), 116–122.

Sasmitaloka, K. S., Rahayuningsih, M., & Sunarti, T. C. (2022). Utilization of Agroindustrial by Product for Bioinsecticide Production. Proceedings of the International Conference on Radioscience, Equatorial Atmospheric Science and Environment and Humanosphere Science, 275, 507–515. https://doi.org/10.1007/978-3-642-03503-6

Sasmitaloka, K. sanggrami, Sunarti, T. C., & Rahayuningsih, M. (2016). Produksi Bioinsektisida Oleh Bacillus thuringiensis subs. aizawai Pada Kultivasi Media padat Menggunakan Limbah Agroindustri. Jurnal Penelitian Pascapanen Pertanian, 13(1), 1–10.

Show, P. L., Oladele, K. O., Siew, Q. Y., Zakry, F. A. A., Wei Lan, J. C., & Ling, T. C. (2015). Overview of Citric Acid Production from Aspergillus niger. Frontiers in Life Science, 8(3), 271–283. https://doi.org/10.1080/21553769.2015.1033653

SNI. (1992). Cara Uji Makanan dan Minuman (SNI 01-289). Badan Standarisasi Nasional.

Soedirga, L. C., Cornelia, M., & Vania. (2018). Analisis Kadar Air, Kadar Serat Dan Rendemen Tepung Singkong dengan Menggunakan Berbagai Metode Pengeringan. Sains dan Teknologi. FaST Jurnal Sains dan Teknologi. 2(2), 8–18. https://ojs.uph.edu/index.php/FaSTJST/article/download/1322/pdf

Styawati, Nur, E., Muhtarudin, Liman. (2014). Pengaruh Lama Fermentasi Trametes Sp. Terhadap Kadar Bahan Kering, Kadar Abu, dan Kadar Serat Kasar Daun Nenas Varietas Smooth Cayene. Jurnal Ilmiah Peternakan Terpadu. 2(1), 19-24. http://dx.doi.org/10.23960/jipt.v2i1.p%25p

Sugiwati, S., Suhartono, M. T., Hanafi, M., & Lioe, H. N. (2018). Produksi β-Glukosidase Aspergillus niger Bio 2173 dengan Fermentasi Padat menggunakan Substrat Dedak. Jurnal Selulosa, 8(1), 33–42.

Suryani, H. F., N. Luthfi. 2022. Evaluasi Kualitas Nutrisi Dedak Padi Dari Pemasok

Bahan Pakan Di Kabupaten Semarang. Journal of Animal Center (JAC)., 4(1) 26- 32. DOI: https://doi.org/10.36378/jac.v4i1.2189

Syamsuriputra, A. A., Setiadi, T., Kushandayani, R., & Yunus, R. F. (2006). Pengaruh Kadar Air Substrat dan Konsentrasi Dedak Padi pada Produksi Asam Sitrat dari Ampas Tapioka Menggunakan Aspergillus niger ITBCCL74. Prosiding Seminar Nasional Teknik Kimia Indonesia, 1–8.

Ul-Haq, I., Ali, S., Qadeer, M. A., & Iqbal, J. (2002). Citric acid fermentation by mutant strain of Aspergillus niger GCMC-7 using molasses based medium. Electronic Journal of Biotechnology, 5(2), 125–132. https://doi.org/10.2225/vol5-issue2-fulltext-5

Ummi, N., & Aviantara, D. B. (2019). Waste Exchange Limbah Onggok Tapioka dengan Proses Biologik untuk Periptaan Polyunsaturated Fatty Acid. Jurnal Rekayasa Lingkungan, 12(2), 136–154.

Vandenberghe, L. P. ., Rodrigues, C., de Carvalho, J. ., Medeiros, A. B. ., & Soccol, C. . (2017). Production and Application of Citric Acid. Current Development in Biotechnology and Bioengineering, 557–575.

Published
2023-10-26
How to Cite
Sasmitaloka, K. S., & Putri, M. D. K. (2023). Added Value Improvement by Cassava Dregs and Rice Bran as Substrates for Citric Acid Production. Food Scientia: Journal of Food Science and Technology, 3(2), 146–164. https://doi.org/10.33830/fsj.v3i2.5106.2023